Replication-Coupled Repair of Crotonaldehyde/Acetaldehyde-Induced Guanine−Guanine Interstrand Cross-Links and Their Mutagenicity†
نویسندگان
چکیده
منابع مشابه
Mechanism of Replication-Coupled DNA Interstrand Crosslink Repair
DNA interstrand crosslinks (ICLs) are toxic DNA lesions whose repair occurs in the S phase of metazoans via an unknown mechanism. Here, we describe a cell-free system based on Xenopus egg extracts that supports ICL repair. During DNA replication of a plasmid containing a site-specific ICL, two replication forks converge on the crosslink. Subsequent lesion bypass involves advance of a nascent le...
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Saccharomyces cerevisiae (budding yeast) cells defective for the PS02/SNM1 gene are uniquely sensitive to agents that produce DNA interstrand cross-links (ICLs), but not other forms of DNA damage. In the absence of Pso2, repair is stalled after the initial incision of ICLs, resulting in an accumulation of DNA double strand breaks (DSBs). The Pso2 protein is a member of the (3-CASP metallo-P-lac...
متن کاملGene-specific DNA repair of interstrand cross-links induced by chemotherapeutic agents can be preferential.
The gene-specific formation and repair of interstrand cross-links (ICL) were measured in the dihydrofolate reductase (DHFR) gene in hamster cells. Cells were treated with two different chemotherapeutic agents, nitrogen mustard and cisplatin, and the frequency of cross-links was quantified in the active gene and in a downstream, inactive region. About 5% of total lesions induced by these agents ...
متن کاملRepair of interstrand cross-links in DNA induced by psoralen plus light.
The clinical drug psoralen, in the presence of 360-nm light, binds to cellular DNA, forming monofunctional adducts with pyrimidine bases and interstrand cross-links. Cross-links, however, rather than monoadducts, appear to be the DNA lesion primarily responsible for the sensitizing action of this drug. Formation of an interstrand cross-link necessarily damages both DNA strands at nearly the sam...
متن کاملDNA repair defects channel interstrand DNA cross-links into alternate recombinational and error-prone repair pathways.
The repair of psoralen interstrand cross-links in the yeast Saccharomyces cerevisiae involves the DNA repair groups nucleotide excision repair (NER), homologous recombination (HR), and post-replication repair (PRR). In repair-proficient yeast cells cross-links induce double-strand breaks, in an NER-dependent process; the double-strand breaks are then repaired by HR. An alternate error-prone rep...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2006
ISSN: 0006-2960,1520-4995
DOI: 10.1021/bi060792v